Thermophilic Fungi with Glucosidase and Proteolytic Activities

The directed search for extremophilic producers in order to obtain hydrolytic enzymes with increased thermal stability has an unconditional practical potential use the food and feed industry improve quality of final product. aim work was study ability collection strains thermophilic fungi show α-L-rhamnosidase, α-galactosidase, cellulase, β-mannanase, keratinase caseinolytic activity. Methods. Micromycetes were grown under submerged conditions test tubes at 42°C 8–14 days. Enzymatic activities studied culture liquid supernatant. p-Nitrophenyl-α-D-galactopyranoside, naringin, guar gum galactomannan Na-carboxymethylcellulose used as substrates determine β-mannanase cellulase activities, respectively. Casein crushed defatted feathers served determination proteolytic Results. enzymatic activity 50 micromycetes belonging 17 species investigated. group showed high activity: 94% had least one, 34% – two, 26% from three five enzyme activities. most active Thielavia terrestris 1920 62, Rhizomucor tauricus 1909, Chrysosporium thermophilum 2050, Thermoascus thermophilus 92 aurantiаcus 2052 (10–26 U/mL). highest α-L-rhamnosidase observed T. 62 (0.35 U/mL), carboxymethylcellulase −in Thermomyces lanuginosus 2046. Six α-galactosidase (0.05–0.2 U/mL) four − (5–130 Conclusions. As a result new producing glycolytic isolated among micromycetes. Soil can be enzymes. Of particular interest are cultures Acremonium 1963, Corynascus C. sepedonium 1899 65068, 1946, which capable complexes proteases glycosidases liquid. This indicates that these promising destructors various technologies processing complex raw materials.